Abstract:Objective: This study aimed to develop novel soybean protein isolate （SPI）-based emulsifiers. Methods: The structure of SPI was modified by limited enzymolysis combined with glycosylation and the effects of this synergistic modification on the emulsification characteristics of SPI was studied. Results: Among the different components obtained from the SPI hydrolysate （SPIH）, the high molecular mass component （F30） exhibited the best emulsifying property. The emulsifying stability of F30-dextran conjugates obtained for 4 h was the best. Compared with SPI, SPIH and F30, the F30-dextran conjugates stabilized emulsions showed the lowest initial average particle size and the best storage stability. When the pH was close to the isoelectric point of SPI or the system was at a high salt concentration, all emulsions were unstable to cause aggregation. Compared with SPI, both SPIH and F30 stabilized emulsions had a higher aggregation degree. However, F30-dextran conjugates provided additional steric hindrance and hydrophilicity due to covalently bound dextran, which exhibited a higher resistance under adverse environmental conditions. Conclusion: Limited enzymolysis combined with glycosylation is a potential and reliable way to develop SPI-based emulsifying ingredients.

Abstract:Objective: Exploring the optimal process for the degradation of T-2 toxin in beer by glow discharge plasma（GDP） and its impact on the physicochemical indicators of beer. Methods: Based on single-factor experiments, a response surface optimization experiment with four factors and three levels was conducted using the Box Behnken method to determine the optimal degradation conditions for T-2 toxin in beer. Results: When the discharge voltage was 570 V, the action time was 18 minutes, the discharge current was 99 mA, and initial concentration of T-2 toxin was 8.5 μg/mL. Under the control of these conditions, the degradation efficiency of the T-2 toxin was the highest （89.21%）. After GDP treatment, the physical and chemical indicators of beer were tested, and the results showed a significant decrease in beer foam retention （P<0.05）, while other indicators remained unchanged. Conclusion: The optimal degradation conditions of GDP obtained by the response surface optimization model are accurate and reliable, which can be used for the degradation of T-2 toxin in beer. GDP can affect the brewing ability of beer, but it will not have a significant impact on other indicators.

Abstract:Objective： To improve the adsorption of genistein by zirconium-based metal-organic frameworks （MOFs） with triangle aromatic ligands. Methods： Two MOFs （MOF-808 and PCN-777） containing different sizes of triangle aromatic ligands were synthesized by hydrothermal method for adsorption of genistein. The synthesis of MOFs was determined by a series of characterization methods, and the adsorption performance was compared with that of a linear binary carboxylic acid ligand-constructed MOF （UiO-66）, to evaluate the effects of pore characteristics, hydrophobicity changes, and other factors on the adsorption performance, and to explore the adsorption mechanism by XPS analysis. Results： Compared with linear ligand-constructed UiO-66, the pore sizes of triangular aromatic ligand-constructed MOFs （MOF-808, and PCN-777） increased from 0.65 nm to 1.81 nm and 3.55 nm, respectively, and the water contact angles increased from 47.91° to 110.68° and 128.23°, respectively. The adsorption capacity and adsorption efficiency of genistein in linear ligand-constructed UiO-66 was 40.08 mg/g and 39.98%, respectively, while the adsorption capacity and adsorption efficiency of genistein in MOFs constructed by triangular aromatic ligand （MOF-808, PCN-777） was increased to 61.80 mg/g, 81.75 mg/g and 61.63%, 81.52% respectively. Conclusion： Metal-organic frameworks （MOFs） can be used for the adsorption and enrichment of genistein. Compared with linear ligands, the introduction of triangular aromatic ligands with different sizes changes the pore size and hydrophobicity of MOFs, enhances the internal accessibility of MOFs, and provides more adsorption sites, which improves the adsorption effect of genistein. The adsorption mechanism of genistein in PCN-777 is based on the synergistic combination of metal-chelating interactions, π-π interactions, and hydrophobic interactions.

Abstract:Objective: In this study, the effects of silymarin on the biofilm S. aureus ATCC25923 and its virulence factors were investigated. Methods: The inhibition and clearance of silymarin on S. aureus ATCC25923 biofilm formation were detected by crystal violet staining, the effects of silymarin on its biofilm metabolism were investigated by MTT staining, and the effects of silymarin on its coagulase expression and hemolytic activity were investigated by freeze-dried plasma and aseptic defibrinated sheep blood. In parallel, the effects of different concentrations of silymarin on its biofilm and Fourier transform were observed under an optical microscope. Results: The results showed that the minimum inhibitory concentration (MIC) of silymarin against S. aureus ATCC25923 was 0.5 mg/mL, the inhibition rate of silymarin to S. aureus ATCC25923 biofilm at MIC concentration reached 90%, and the clearance rate reached 89.2%, and the low dose of silymarin could significantly inhibit its hemolytic activity and coagulase expression. Conclusion: The outcomes of the experiment demonstrate the great potential applications of silymarin in the areas of natural preservatives and food safety.

Abstract:Objective: Obtaining hhigh-yield pprotease-pproducing bacterial resources through fermentation of advantageous protease-producing strains derived from Shiping stinky tofu. Methods: Single-factor and response surface experiments were used to optimize the culture medium and fermentation conditions. Using casein as the substrate, the effects of temperature, pH, metal ions and organic reagent on the protease activity and stability of strain JX-11 were investigated using enzymology techniques. Results: One strain of high-producing proteinase-secreting bacteria, JX-11, was isolated from Shiping stinky tofu, identified as Chryseobacterium pennipullorum. The optimal conditions for protease production by JX-11 strain were found to be temperature at 23.0 ℃, glucose content at 6.7 g/L, peptone content at 15.0 g/L, pH 6.4. Under these conditions, the protease activity was (39.16±3.24) U/mL. The optimal temperature for extracellular protease of JX-11 strain was 30 ℃, and it had good stability in the range of 10～40 ℃. The optimal pH was 7.0, and it had good stability in the range of pH 6.0～9.0. Mn²⁺ significantly increased the activity of JX-11 protease. The relative activity was increased by 4.33 times compared with the blank group. Zn²⁺, Cu²⁺, and K⁺ all inhibited the enzyme activity, Na⁺, Mg²⁺, and Ca²⁺ had no significant effect. Glycerol can promote the activity of the enzyme, but ethanol and acetone have little effect on it. Tween 80, acetic acid, methanol and EDTA inhibited the activity of the enzyme, among which EDTA hadd the most obvious inhibition, which further proved that the enzyme was a metalloprotease. Conclusion: A high-producing protease-secreting bacterial strain is obtained from Shiping stinky tofu, which has good application prospects.

Abstract:Objective: To study the molecular mechanism of celery seed inhibiting or treating gout by using network pharmacology and molecular docking. Methods: TCMSP database and the network maps were constructed with Cytoscape3.9.0 software. Gout-related targets were collected in GeneCard database and OMIM database, crossed with the main component targets of celery seed and imported into STRING database for protein-protein interaction （protein-protein interaction, PPI） analysis, core targets were obtained, core targets were introduced into DAVID, and GO function enrichment and KEGG pathway enrichment were performed in the database. Finally, AutoDock software was used to verify the key components with key targets. Results: There were 16 main components of celery seeds, 69 common targets. After topological analysis, quercetin, apigenin, luteolin, celery, five core components and 10 key targets of gout TNF, MAPK14, IL4, CXCL8, LYN, PDGFRβ, HCK, VEGFA, ITGA, IL2, were closely involved in inhibiting or treating gout. The results of GO and KEGG analysis showed that the core components of celery seed acted through regulating biological processes such as apoptosis, proliferation and migration, and through PI3K-Akt signaling pathway, IL-17 signaling pathway, MAPK signaling pathway, and NF-κB signaling pathway. The molecular docking results showed that the five core components were closely bound to the 10 key target proteins with high confidence, verifying the accuracy of the network pharmacology prediction results. Conclusion: This study reveals the role of celery seed in inhibiting or treating gout, and lays a foundation for the development of single Chinese herbal medicine and the study of "medicine and food homology" to control metabolic diseases.

Abstract:Objective: Improve the accuracy and robustness of papaya ripeness detection. Methods: A method of papaya ripeness detection based on multi-target sampling and improved Mask R-CNN was proposed. In the process of data expansion, the method introduced multi-object sampling technology to generate enhanced images from small data sets taken under controlled conditions, which was conducive to extending the proposed method to data sets with complex features of actual papaya images. The effectiveness and robustness of the proposed method were verified by means of average accuracy, accuracy, accuracy-recall curve and calculation time, and the results of papaya ripeness detection effect were compared with those of Faster R-CNN, RetinaNet and CenterMask. Results: The values of mean awerage precision, 50% mean awerage precision and 75% mean awerage precision for the papaya ripeness detection were 98.43%, 98.67% and 98.68%, respectively. The average accuracies for the ripeness detection of immature, semi-mature and mature papayas were 99.38%, 98.81% and 99.37%, respectively. Conclusion: This method can be used to develop an electronic system for papaya ripeness detection and improve the performance of papaya ripeness detection and grading.

Abstract:Objective: The objective of this study is to investigate the heavy metal content in sediment and various organs of Hunan lotus seed in a planting area, as well as to explore the characteristics of heavy metal accumulation and transfer in Hunan lotus seed. Methods: A typical planting area in Hunan Province was selected for this research. The contents of Cd, Cr, Pb, Ni, Cu, Zn and Mn were measured using an inductively coupled plasma emission spectrometer. The bioconcentration factor （BCF） and transfer factor （TF） were used to evaluate the heavy metal accumulation and transfer ability of Hunan lotus seed. Results: The average content of Cd, Cr, Pb, Ni, Cu, Zn, and Mn in the sediment were 0.73, 176.28, 40.48, 27.61, 28.07, 95.57 and 311.68 mg/kg, respectively. Compared with the limit values of NY/T 391—2021, the exceeding rates of Cd, Cr, and Pb content are 100%, 75%, and 10%, respectively. The highest concentrations and contents of these 7 heavy metals were Cd, Cu and Zn in the lotus core, Cr and Pb in the root, Ni in the lotus-seed kernel and Mn in the leaves, with an average content of 0.20, 13.64, 78.58, 14.66, 2.23, 1.11 and 924.03 mg/kg, respectively. The BCF_Mn values for both stems and leaves exceeded 1. The TF_Cu values for all organs were greater than 1. Conclusion: This study reveals that there is serious pollution from Cd within the study area. The content of heavy metals in different organs of Hunan lotus seed is different. Lotus stems and leaves has strong enrichment of Mn, and Cu had strong transfer ability in Hunan lotus seed.

Abstract:Objective: To achieve rapid, quantitative and high-throughput detection of aminoglycoside antibiotic residues in pork. Methods: Square-array aligned SERS substrates based on Au nanoflowers （AuNFs） were prepared using PP synthetic paper as a substrate. Pork samples were pre-treated by QuEChERs method and subjected to SRES. Results: Using 4-mercaptobenzoic acid as the SERS probe molecule, the substrate exhibited good homogeneity, SERS enhancement effect, reproducibility and stability. the SERS signal intensities at the characteristic peaks at 475, 619 cm^-1 showed good linear relationships with the logarithms of the concentrations of gentamicin sulphate and neomycin sulphate, respectively （R² of 0.991 6 and 0.990 7, respectively）. The limits of detections （LODs） were as low as 1×10^-9, 1×10^-8 mol/L, respectively, and were successfully applied to the rapid, quantitative and high-throughput determination of aminoglycoside antibiotics in pork. Conclusion: The experimental method provides an economical, efficient, time-saving and highly sensitive way for the application of SERS technology for the detection of antibiotic residues in real meat samples.

Abstract:Objective: This study aimed to evaluate the determination uncertainty of four quinolones residues in honey by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. Methods: Based on GB 31657.2—2021, a mathematical model for sources of uncertainty evaluation was established. The uncertainty of each variable which affect the result were calculated and the weight of each uncertainty component was assessed. Results: The uncertainties in the determination of enrofloxacin, sarafloxacin, ofloxacin and ciprofloxacin in honey were （4.97±0.81）, （10.13±1.58）, （7.67±1.23）, （14.97±2.25） μg/kg （P=95%，k=2） respectively. Conclusion: The main influencing factors of the determination uncertainty were preparation and dilution process of standard solution and fitting of standard curve, which should be controlled in practical work.

Abstract:Objective: A method for the determination of 7 synthetic colorants in freshly prepared beverages by high performance liquid chromatography dual （HPLC） wavelength method with automatic solid phase extraction was established. Methods: Extraction conditions: The sample was extracted 2 times with pure water, and then the supernatant combined after centrifugation. The pH of the supernatant was adjusted to 3~4. After activation by an automatic solid-phase extraction device, loading, rinsing, and elution of the sample were carried out on the Poly-sery synthetic colorant solid-phase extraction column. The eluent was concentrated to 200 μL by nitrogen blowing, and then fixed to 1.0 mL with 50% mobile phase A+50% mobile phase B. Instrument conditions: The column was C₁₈ column. The mobile phase was 20 mmol/L ammonium acetate-methanol, gradient elution. The flow rate was 1.0 mL/min. The injection volume was 10 μL. The detection wavelength were 254 nm and 628 nm. Results: The correlation coefficient of each substance in the range of 0.5～50 μg/mL was greater than 0.999 9, the detection limit of the method was 0.023～0.179 mg/kg, the quantitative limit was 0.078～0.598 mg/kg, the standard recovery was 92.4%～96.8%, and the relative standard deviation （RSD） was 1.2%～4.2%. Conclusion: This method has low consumption of toxic reagents, simple operation steps, high degree of automation, good recovery and repeatability, and it is suitable for the determination of large quantities of samples.

Abstract:Objective: Taking Guangxi Liupao tea as an example, an UPLC-MS/MS analysis method with residual amounts of matrine and Oxymatrine was established. Methods: The prepared samples were subjected to optimized acetonitrile aqueous solution （80% acetonitrile+0.2% ammonia water） ultrasonic extraction, mobile phase: formic acid water and formic acid methanol （both 0.1%）, gradient elution, and internal standard method quantification. Results: It was linear in the range of 0.1～80.0 ng/mL, r was greater than 0.999 6, the detection limits of matrine and oxymatrine were 1.0 μg/kg, and the quantitative limits were 3.0 μg/kg, which met the regulatory determination requirements for the residue of matrine and oxidative matrine in tea. Through the spike verification, the recovery rate was in the range of 92.0%～104.7%, and the relative standard deviation （RSD） was less than 5.4%. Conclusion: Method combined with purification tube purification （147.7 mg PSA, 15.1 mg GCB, 887.2 mg magnesium sulfate）, using Kinetex^ 2.6 μm Biphenyl 100  chromatographic column （100 mm×3.0 mm） separation solves the problems of low extraction recovery rate and complex pre-treatment of matrine and oxymatrine, so that the symmetrical peak shape and strong anti-interference ability of matrine and oxymatrine.

Abstract:Objective: To analyze and evaluate the uncertainty of the determination of folic acid content in milk powder by microplate method. Methods: The content of folic acid in infant formula milk powder was determined by microplate method according to GB 5009.211—2022 "National Standard for Food Safety-Determination of Folic Acid in Foods". Various experimental factors affecting the test results were analyzed and calculated according to the uncertainty evaluation methods and guidelines such as JJF 1059.1—2012, CNAS-CL01-G003:2011 and CNAS-GL05:2011, and the synthetic standard uncertainty and expanded uncertainty were obtained. Results: The expanded uncertainty U₉₅=22.08 μg/100 g（k=2）, and the folate content of the sample was （282.33±22.08） μg/100 g. The uncertainty mainly comes from the process of microplate inoculation, the fitting of standard curve and the repeatability of measurement. Conclusion: The accuracy of pipettes and microbial factors have a great impact on the experimental results, so it is necessary to ensure the quality of the instrument and strictly follow the standardized procedures to minimize the uncertainty.

Abstract:Objective： A surface evaporation concentration device were designed to concentrate thermosensitive feed liquid at low temperature under normal pressure with low energy consumption. Methods： Based on an introduction to the working principle of the device, the basic equations of the device were given. The changes of volumetric evaporation flux and energy saving ratio with flow rate of the feed liquid, feed liquid temperature, sweeping gas flow rate, sweeping gas temperature were calculated and analyzed. Designed an experimental platform and obtained a set of optimal performance parameters through experiments. Results： The results showed that when the flow rate of the feed liquid was 0.02 kg/s, the device had the highest energy saving ratio and higher volumetric evaporation flux; When the temperature of the feed liquid increased from 30 ℃ to 50 ℃, the volumetric evaporation flux increased by 224.68%, but the energy saving ratio decreased by 12.42%; When the sweeping gas flow rate increased from 0.004 kg/s to 0.006 kg/s, the volumetric evaporation flux increased by 23.45%, but the energy saving ratio decreased by 7.74%; When the temperature of the sweeping gas increased from 10 ℃ to 30 ℃, the volumetric evaporation flux decreased by 22.92%, but the energy saving ratio increased by 37.49%; An experimental device was established to test orange juice with a sugar content of 15 °Brix, the results showed that when the feed liquid temperature was 38 ℃ and the sweeping gas temperature was 26 ℃, the volumetric evaporation flux of the device could reach 219 kg/（m³·h）, and the energy saving ratio could reach 3.3.Conclusion： The heat pump assisted random filler surface evaporation device has the characteristics of low temperature concentration under atmospheric pressure and low energy consumption when concentrating thermosensitive feed liquid．

Abstract:Objective: To optimize the operating parameters of wheat milling in roller mill I skin mill to reduce the energy consumption of milling. Methods: Using EDEM discrete element simulation software, Box-Behnken test was designed for key milling factors such as roll pitch, feeding amount, rotational speed and rotational speed ratio to investigate the relationship between operating parameters and milling power. Results: The significance rankings of the effects on the toothed roll crushing power were, in order, speed ratio, roll pitch, feed volume, speed ratio quadratic term, roll pitch quadratic term, interaction of roll pitch and speed ratio, interaction of feed volume and speed ratio, and interaction of roll pitch and feed volume. According to the different requirements of the I-skin mill, the optimal parameter combinations were obtained: the finer powder output with a rolling pitch of 0.67 mm, a feeding amount of 803.60 kg/（cm·d）, a fast roller speed of 537.68 r/min, and a rotational speed ratio of 2.64, and the coarser powder output with the optimal low-power consumption of 0.79 mm, a feeding amount of 803.83 kg/（cm·d）, a fast roller speed of 576.08 r/min, and a rotational speed ratio of 2.23. Conclusion: The error between the simulation results and the measured results is within a reasonable range, and the established discrete element milling model can be used for power prediction in wheat milling.

Abstract:Objective: To solve the problems of low level of automation and high labor intensity of workers in the fermented grains discharging process of solid-state fermentation in the ground-pot. Methods: An automatic fermented grains scooping strategy for the compound robot for fermented grains scooping was proposed. Based on the pre-processing of the ground-pot image and the reconstruction of the point cloud inside the inner edge, the point cloud segmentation algorithm was used to extract the point cloud of fermented grains. The scooping plane was constructed. The safe working distance for scooping was solved. Preset scooping points were set. Afterwards, the scooping amount of the preset scooping point was evaluated by the pixel surface element voxel algorithm, to select the optimal scooping point. Results: The method of planning the scooping point based on the safe working distance is reasonable, which ensures that the bucket does not collide with the ground-pot wall while scooping fermented grains from the wall at different depths of the scooping plane. The strategy can effectively reduce the number of robot scooping, thereby improving the efficiency of fermented grains scooping. Conclusion: The strategy can effectively guide the compound robot to realize the automatic and intelligent fermented grains discharging.

Abstract:Objective： This study aimed to solve the problems of yellowing, discoloration and quality preservation of broccoli after harvesting. Methods： Broccoli was fumigated with chlorine dioxide gas at different mass concentrations (0,0.2, 0.5, 1.0, 1.5, 2.0 mg/L) at 23 ℃, and the sensory, color, yelination grade, physiological and biochemical indexes and related browning enzyme activities of broccoli were determined after 0,2,4,6,8 days of storage. Results： Compared with the control group, different mass concentrations of ClO₂ could inhibit the mass loss rate, yellowing browning and spoilage of flower buds of broccoli. ClO₂ at 0.5 and 1.0 mg/L had a more significant effect on the maintenance of chlorophyll content, color difference a^* and b^*, and the inhibition of PPO and POD enzyme activities in broccoli. Among them, 0.5 mg/L ClO₂ had the best effect on maintaining the color difference value of broccoli flower buds, and the yelination grade was still grade Ⅱ on the 8th day of storage. Simultaneously, it effectively maintained the vitamin C content and delayed the yelination and Browning of flower buds. Conclusion： It is necessary to control the concentration of ClO₂ gas in the fumigation treatment of broccoli to obtain the best effect of maintaining the fresh green of broccoli bulbs and extending the shelf life.

Abstract:Objective: A new type of natural food preservative with pork liver as the main raw material was developed. Methods: Water-soluble porcine liver protein (WSLP), salt-soluble porcine liver protein (SSLP) and chitosan (CS) were combined to create edible composite membranes. The edible membrane components were then examined from a variety of perspectives using mechanical properties, Fourier transform infrared spectroscopy (FT-IR) and microstructure analysis. Utilized to preserve pork, and by contrasting the changes in the physical and chemical indices during the chilling process, the freshness retention capabilities of the composite film were investigated. Results: With the addition of WSLP and SSLP, the mechanical properties of edible film showed a trend of increasing firstly and then decreasing. The film was best when the amount was 40%, its film thickness was (0.267±0.001 43) and (0.264±0.001 21) mm, the tensile strength was (9.63±0.29) and (4.43±0.37) MPa, and the elongation at break was (58.37±0.90)% and (28.24±0.63)%, respectively, which were significantly different from CS film (P<0.05), and the FT-IR results showed that it was 40% WSLP and SSLP maximize cross-linking between membrane matrices. The results of scanning electron microscopy showed that the surface of the composite film prepared by 40% WSLP and SSLP was smooth and flat, and the microstructure was the best. After refrigerating pork for 12 days; It was found that 40% SSLP composite film had the best preservation performance, and the pH, total number of colonies, TVB-N value and TBARS value were the smallest in the four groups under different treatments. Conclusion: The composite membranes prepared by 40% WSLP and SSLP have the best performance.

Abstract:Objective: The preservation effects of four packaging materials on passion fruit were studied. Methods: The fruit of passion flower cv. Huangjin （maturity: eight） was used as the research material, and passion flower fruit was treated via polyvinyl chloride （PVC） film, polyethylene （PE） bag, bidirectional stretch polyolefin （BSP） heat shrinkable film, biodegradable modified atmosphere （BMA） bag, respectively, with untreated ones as the control group. Then, the fruits was stored at temperature of （25±1） ℃ and relative humidity of 85% for fifteen days, and the preservation indices of fruit were measured every three days. Results: Compared to control passion fruit, the four treatments of packaging materials could delay the peak of respiration and ethylene, slow down the increase of pericarp malondialdehyde content to stabilize cell membrane structure, delay the fruit etiolation and shrinkage, keep a lower weight loss rate and a higher commercially acceptable fruit rate, and maintain the pericarp appearance color and pulp nutrients contents. Among the different packaging materials, BSP heat shrinkable film had the best effects. Conclusion: The BSP heat shrinkable film might be used as a suitable storage technology to improve the quality and prolong the storage life of harvested "Huangjin" passion fruit.

Abstract:Objective: This study aimed to demonstrate the potential immunomodulatory activity in mice of rice immunopeptide GBP1 （NSVFRALPVDVVANAYR） screened previously. Methods: Animal experiments were conducted to investigate the immunomodulatory mechanism of GBP1 peptide. RNA-Seq was used to sequence the transcriptome of mouse spleen tissues before and after GBP1 peptide treatment, and the molecular mechanism of GBP1 peptide immunomodulatory effect was explained. Results: In the GBP1 peptide treatment group, the number of spleen cells tended to be normal, the liver organ coefficient decreased significantly, and the serum IgG content was stable.GBP1 peptide can affect the expression of cytokines, mediate signal transduction pathways, and exercise immune regulation function, and inhibit the secretion of IL-2, IFN-γ and TNF-α in serum of mice. Through transcriptome analysis, 780 differentially expressed genes were obtained from spleen tissue, which were closely related to immune-related p53 signaling pathway, ferroptosis, complement and coagulation cascade in the body. Conclusion: GBP1 peptide can affect the expression of cytokines, mediate signal transduction pathways, and exercise immune regulation function.

Abstract:Objective: To explore the effects of Brassica rapa L. acid polysaccharide 1 (BRAP-1) on H226 cell tumor-bearing mice. Methods: 60 mice were divided into the model group (0.1 mL/10 g), positive cisplatin [3 mg/(kg·2 d)], BRAP-1 low dose [50 mg/(kg·d)], BRAP-1 medium dose [100 mg/(kg·d)] and BRAP-1 high dose [200 mg/(kg·d)]. During the experiment, the tumor growth of mice was observed, and the tumor inhibition rate and organ index were calculated; The levels of interleukin-18 (IL-18), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α) in serum were detected by enzyme-linked immunosorbent assay. The gene expressions of IL-18, IL-1β, RIP-1(receptor-interacting protein-1), RIP3 and MLKL (mixed-lineage kinase domain-like protein 3) were detected by Real-time Quantitative PCR (q-PCR) and Western blot (WB). Results: Compared with the model group, the low and middle dose of BRAP-1 groups had significant tumor inhibition effects (P＜0.05), and the positive group and the high dose of BRAP-1 group had significant tumor inhibition effects (P＜0.01). Compared with other groups in the model group, the serum levels of IL-1β in the positive group and the high dose of BRAP-1 group were significantly increased (P＜0.01), IL-18 and TNF-α were significantly decreased in the positive group (P < 0.05), and were significantly increased in each dose of BRAP-1 group (P < 0.01). The results of q-PCR and Western blot showed that compared with the model group, the relative expression of IL-1β mRNA decreased with the increase of BRAP-1 dose (P＜0.01), while the relative expression of IL-18, MLKL, RIP1, and RIP3 mRNA and protein increased (P＜0.01). Conclusion: BRAP-1 can inhibit the growth of lung squamous carcinoma H226 cells by regulating the necrotizing apoptosis-related proteins MLKL, RIP1, and RIP3 and regulating immune cytokine levels.

Abstract:Objective: This study aimed to develop antioxidant products and anti-diabetes drugs of wampee. The study aimed to research the structural characteristics, anti-oxidation and hypoglycemic properties in vitro of polysaccharides from different parts of wampee. Methods: The structural properties, in vitro antioxidant and hypoglycemic activities of polysaccharides from the fruit, peel and seeds of wampee (CWP-F, CWP-P, and CWP-S) were analyzed and compared from the aspects of chemical composition, molecular weight, monosaccharide composition, appearance, DPPH free radical scavenging rate and α-amylase inhibition rate. Results: CWP-F had the highest molecular weight and was mainly composed of galactose and arabinose, CWP-P was mainly composed of galacturonic acid and arabinose, and CWP-S was mainly composed of glucose. The absorption peaks of the carboxylic acid structure were found in the Fourier transform infrared spectra of the three polysaccharides. Scanning electron microscopy results showed that the surface structure of the three polysaccharides was fluffy. CWP-P had the strongest antioxidant capacity, and its DPPH free radical scavenging rate, OH free radical scavenging rate, and total reducing power all were higher than those of CWP-F and CWP-S. CWP-P had the strongest hypoglycemic ability, followed by CWP-F, and CWP-S had no hypoglycemic ability. Conclusion: In vitro, CWP-F, CWP-P, and CWP-S exhibited varying compositions, structures, and antioxidant and hypoglycemic activity. Notably, CWP-P has shown the most robust antioxidant and hypoglycemic properties.

Abstract:Objective: This study aimed to determine the extraction solvent and compare the distribution of functional ingredient and antioxidant activities in different parts of Penthorum chinense pursh. Methods: the total phenolic content, total flavonoid content, antioxidant capacity and individual phenolic compounds content in Penthorum chinense pursh were determined and analyzed. Results: The total phenolic content, total flavonoid content increased and then decreased with the increase of solvent concentration. The seven main phenolic compounds （catechin, rutin, pinocembrin-7-O-glucoside, quercetin, kaempferol, pinocembrin and thonningianin A）, ABTS radical scavenging ability, and ferric ion reduction ability were the highest in 80% ethanol extract. The contents of total phenolic content, total flavonoid content, ABTS radical scavenging ability, DPPH radical scavenging ability and ferric ion reducing ability of different parts of Penthorum chinense pursh were flower > leaves > stem. The total phenolic content, total flavonoid content, antioxidant capacity of flower and stem decreased significantly with the increasing of storage time, while leaves were relatively stable. In addition, the antioxidant activities of Penthorum chinense pursh were significantly positively correlated with total phenolic content, catechin, isoquercetin, kaempferol-3-O-rutinoside, afzelin, and pinocembrin-7-O-glucoside （P < 0.01）. Conclusion: 80% ethanol appears the most effective for polyphenol extraction in Penthorum chinense pursh, and the leaves have the great potential for development and utilization with high content and stable antioxidant activity.

Abstract:Objective: Clarify the flavor characteristics of Tremella fuciformis cultivated on herbaceous substrate. Methods: In the study T. fuciformis cultivated on herbaceous substrate was used as the research object, while samples of yellow tremella and white tremella cultivated on conventional substrate were used as controls. The differences in flavor composition between them were analyzed using high-performance liquid chromatography and Headspace Solid Phase Microextraction Ggas Chromatography Mass Spectrometry (HS-SPME-GC-MS). And combined with intelligent sensory technology, its flavor characteristics were analyzed. Results: According to multi-dimensional evaluations such as fresh and sweet amino acid and flavor nucleotides content, taste activity value and equal freshness concentration value, and electronic tongue, the freshness intensity of this herb's tremella was lower than that of yellow tremella and white tremella. The results of HS-SPME-GC-MS analysis showed that 82 volatile compounds were identified in this herb Tremella, which were higher than those in white Tremella (71 species) and yellow Tremella (49 species). The main compounds were esters, accounting for 16.9%～22.4%, presenting a strong floral aroma. At the same time, 26 characteristic aroma components of Tremella, 9 unique components of white Tremella, and 4 unique components of this herb Tremella were identified; In terms of aroma substance content, this herb Tremella also holds an advantage. Conclusion: The traditional Chinese medicinal fungus has a light taste and a strong aroma, making it suitable for developing and preparing beverages, while yellow white fungus is more suitable as a dish.

Abstract:Objective: The difference in bioactive substance content and bioactivity in black beans were compared. Methods: The contents of total phenolic acids, total flavonoids, condensed tannins, total anthocyanins, total polysaccharides and total proteins of nine kinds of black beans were determined. The antioxidant activities of black bean extracts in vitro and their inhibitory effects on α-amylase, pancreatic lipase, acetylcholinesterase and tyrosinase were compared. Results: The contents of total phenolic acid, total flavonoids, condensed tannins and total anthocyanins were as follows: equine bean > small black bean > black bean; Total polysaccharide content: small black bean > black bean > horse bean; Total protein content: horse bean > black bean > small black bean. Antioxidant activity: horse bean > small black bean > black bean. In vitro experiments showed that little black bean and black bean had inhibitory effects on many enzymes, and the intensity was different. Conclusion: The contents and functions of bioactive substances in different varieties of black beans are different. Black beans are more suitable for eating, and horse bean and small black beans have higher medicinal value.

Abstract:Objective： Based on transcriptome sequencing analysis, the effect of grape seed procyanidins on HepG2 cell genes and related functions was identified. The key genes and metabolic pathways involved in the treatment of HepG2 cells with procyanidins were confirmed. Methods: Through transcriptome sequencing, screening differential genes, gene functional annotation and enrichment analysis of the KEGG pathway, transcriptome studies were conducted on grape proanthocyanidin-treated cells. Results: After treating HepG2 cells with grape anthocyanins, the main biological processes enriched are cellular processes, metabolic processes, biological regulatory processes, immune system processes, reproductive regulation, and growth regulation. Research has found that 12 key differentially expressed genes in cell apoptosis are associated with TNF, p53, MAPK, PI3K-Akt and NF-κB signaling pathway is closely related. Conclusion: Cell apoptosis is closely related to the TNF signaling pathway, p53 signaling pathway, PI3K/Akt signaling pathway, NF-κB signaling pathway and MAPK signaling pathway.

Abstract:Objective: This study aimed to explore the copigmentation formula of purple corn anthocyanins. Methods: In this study, fructose, tannic acid, and pectin were used as co-pigmentation. The response surface method was used to optimize the co-pigmentation formula of purple corn anthocyanins, and the untreated purple corn was used as a control test. The anthocyanin components, color, texture, taste and antioxidant activity (DPPH free radical scavenging ability, ABTS free radical scavenging ability, and FRAP iron ion reducing ability) of purple corn after different treatments were determined. Results: 19% fructose, 0.07% tannic acid, and 1.9% pectin had the best copigmentation effect. Compared with the control group, the contents of cyanidin-3-O-glucoside, geranium-3-O-glucoside, and peony-3-O-glucoside in purple corn increased by 77.64%, 64.82%, and 54.75%, respectively. The total anthocyanin content increased by 67.98%. The L^* value, b^* value, and ΔE value of color decreased, while the a^* value increased. Hardness, adhesiveness, and chewing type increased, while elasticity and cohesion decreased. The bitter, astringent, and astringent aftertastes differ slightly, as do the sour, sweet, salty, umami, bitter, and umami aftertastes. In the mass concentration range of 0.02~0.10 mg/mL, the DPPH free radical scavenging ability, ABTS free radical scavenging ability and FRAP iron ion reducing ability of purple corn anthocyanins were 39%~79%, 54%~74% and 27%~67%, respectively. Conclusion: The co-pigmentation effect of 19% fructose, 0.07% tannic acid, and 1.9% pectin on purple corn anthocyanins was the best.

Abstract:Objective: To Prepared of β-Cyclodextrin inclusion complex with Foeniculum vulgare（fennel） volatile ooil and study on its sstructural characterization and stability. Methods: Based on the inclusion rate, the inclusion yield and the content of trans-anethole in the inclusion compound, the comprehensive evaluation index was established, and the effects of the ratio of fennel volatile oil to β-cyclodextrin, inclusion temperature and inclusion time on it were explored. The preparation process was optimized by orthogonal test. It was characterized by scanning electron microscopy, thin layer chromatography, Fourier infrared spectroscopy and differential scanning calorimetry. Finally, the stability of the inclusion compound was investigated by light, high temperature and high humidity tests. Results: The optimum conditions obtained by saturated aqueous solution method were as follows: the ratio of volatile oil to β-cyclodextrin was 1∶8 (mL/g), the inclusion temperature was 40 ℃, the inclusion time was 60 min. Under these conditions, the inclusion rate of fennel volatile oil inclusion compound was 96.90%, the inclusion yield was 93. 61%, the content of trans-anethole was 6.81%, and the comprehensive score was 99.68. The inclusion compound was white powder with loose texture and good inclusion effect. The inclusion compound was characterized by scanning electron microscopy, thin layer chromatography, Fourier infrared spectroscopy and differential scanning calorimetry. Its stability was good within 5 days of illumination (4 000 lx), volatile components in the inclusion compound were easy to lose at high temperature (60 ℃) for a long time, and it would deliquesce and agglomerate when the relative humidity was more than 75%. Under the conditions of long illumination time, high temperature and high humidity, the inclusion rate and trans-anethole content were obviously reduced. Conclusion: The inclusion rate and inclusion yield of the above-mentioned optimal inclusion process are high, and the process is stable. When stored in a dry and sealed environment at room temperature, the β-cyclodextrin inclusion compound of fennel volatile oil has certain stability compared with the volatile oil.

Abstract:Objective: This study aimed to explore suitable drying methods for oil tea powder, making it easier for oil tea consumption and storage. Methods: Spray drying, vacuum freeze drying and vacuum drying were used to dry oil tea soup, and the quality (moisture content, sensory evaluation and color) and volatile substances of dried oil tea powder were evaluated. Results: The sensory score of vacuum freeze-drying oil tea powder was the highest (93), the water content was the lowest (3.78%), and the color was better（ΔE value 49.74). 104 volatile substances were identified from three drying methods of oil tea powder using SPME-GC-MS technology, of which 14 affected the flavor of oil tea powder. The characteristic flavor substances (nonanal, decanal, (Z)-2-decanal, (Z)-2-nonenal, (E) -3,7-dimethyl-2,6-octadienal) of freeze-drying present green fragrance and fat fragrance. The special aromas of spray drying and vacuum drying are (Z)-3,7-dimethyl-2,6-octadienal, (E)-3,7-dimethyl-2,6-octadienal and decanal, presenting green fragrance, fruit fragrance, and wax fragrance. PCA and comprehensive evaluation indicate that vacuum freeze-drying has the best flavor quality, which is consistent with sensory evaluation results. Conclusion: The flavor quality of vacuum freeze-drying was the best.

Abstract:In this review, several mechanisms of antihypertensive peptides derived from food and its by-products were summarized. Mechanisms based on the RAAS system such as ACE/Ang Ⅱ/AT1R signaling pathways and ACE2/Ang (1-7)/MasR signaling pathways were included. Mechanisms targeting at KNOS system including PI3K/Akt/eNOS signaling pathway, PPAR-γ/caspase3/MAPK/eNOS signaling pathway and L-type Ca²⁺ channel were also discussed. In addition, the pathways of reducing blood pressure by inhibiting endothelin-converting enzyme (ECE) activity discovered in recent years were also introduced.

Abstract:Steamed bread, noodles, deep-fried dough sticks and other noodle foods are an important part of our diet, and gluten protein is an important component of noodle foods. Common food processing techniques used to prepare noodles, including heating, freezing, mechanical processing, fermentation, and electric field treatment, alter the hydrophobic disulfide bond of the protein, denature and aggregate the protein, and cause other intricate physical and chemical changes, all of which have an impact on the texture and flavor of the finished noodle product. This paper mainly introduces the current research status of the influence of processing methods such as heating, freezing, crushing, kneading, extrusion, fermentation, and electric field processing on the structure and properties of the gluten protein.

Abstract:Pectin is a kind of natural polysaccharide polymer, which is an important part of the cell wall of higher plants. It has physiological functions such as cholesterol-lowering, anti-tumor, anti-oxidation and hypoglycemic, and beneficial to intestinal health. In this review, pectin extraction methods and their advantages and disadvantages in recent years were summarized, and the yield, galacturonic acid content and esterification degree of pectin extracted from different sources and methods were compared and analyzed. The physiological function of pectin and its application in various aspects were also introduced, and the future development direction was prospected.