Abstract:Objective To establish an analytical method for the determination of residues of eight fungicides (ametoctradin, triadimefon, triadimenol, flutriafol, mandipropamid, penthiopyrad, isopyrazam, and prochloraz) in eggs by solid-phase extraction (SPE) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS).Methods The samples are ultrasonically extracted with acetonitrile. The extracts are purified using ProElut DPC solid-phase extraction cartridges. The purified eluate is concentrated under reduced pressure and reconstituted with acetonitrile. Chromatographic separation is performed on a Kinetex C18 column (2.6 μm, 2.1 mm×100 mm) with gradient elution using 0.1% formic acid in water and acetonitrile as the mobile phase. Detection is carried out using electrospray ionization (ESI) in positive ion mode with multiple reaction monitoring (MRM), and quantification is performed using the external standard method.Results The eight fungicides exhibit good linearity within their respective concentration ranges (R2>0.99). The limits of detection (LODs) range from 0.03 to 0.50 μg/kg, and the limits of quantification (LOQs) range from 0.10 to 2.00 μg/kg. The average recoveries range from 81.6% to 107.0%.Conclusion The established method is simple, accurate, and reproducible, making it suitable for the determination of fungicide residues in eggs.