Objective Based on classical prescriptions of Xin'an Medicine, a functional food was developed to help exert antioxidant effects and maintain healthy blood glucose levels.Methods Based on the results of single-factor experiments, an orthogonal design was applied to optimize three factors in the extraction process, namely the solid-liquid ratio, extraction time, and number of extraction cycles. The extraction yields of total polysaccharides and total flavonoids were used as evaluation indices to determine the optimal extraction process. The in vitro antioxidant activity of Suopiyin was evaluated using the scavenging rates of DPPH, ABTS, and hydroxyl (·OH) radicals. The in vitro hypoglycemic activity was assessed by measuring the inhibitory rates of α-glucosidase and α-amylase. A type 2 diabetes mellitus (T2DM) rat model was established. Fasting blood glucose, body weight, 24-h food intake, and water intake were recorded. Blood lipid levels and oxidative stress indicators were measured, and hematoxylin-eosin (HE) staining was used to observe histopathological changes in liver tissue.Results The optimal extraction conditions for Suopiyin were as follows: a solid-liquid ratio of 1:10 (g/mL), an extraction time of 60 min, and two extraction cycles. Under these conditions, the extraction yields of total flavonoids and total polysaccharides were (9.15±0.25) mg/g and (122.76±1.48) mg/g, respectively. The IC₅₀ values of the Suopiyin extract for DPPH, ABTS, and ·OH radicals were (0.492±0.130), (0.669±0.080), and (14.568±2.010) mg/mL, respectively. The IC₅₀ values for inhibition of α-glucosidase and α-amylase were (6.006±0.730) and (6.864±1.060) mg/mL, respectively. These results indicate that the Suopiyin extract prepared under the above process conditions exhibited good antioxidant and hypoglycemic activities in vitro. In vivo experiments showed that rats in the model group had significantly increased blood glucose levels and reduced body weight, accompanied by increased food and water intake. In contrast, the symptoms of polyphagia, polydipsia, and polyuria were markedly alleviated in the Suopiyin group, and blood glucose levels were significantly reduced (P<0.05). The serum TC, TG, and LDL-C levels in the Suopiyin group were significantly lower than those in the model group (P<0.05), whereas HDL-C showed no statistically significant difference compared with the model group. In addition, serum SOD activity in the Suopiyin group was significantly increased, while the MDA level was decreased (P<0.05). Liver pathological damage was alleviated, and the number of lipid vacuoles was reduced. These findings indicate that Suopiyin can reduce blood glucose levels in T2DM rats, improve oxidative damage and dyslipidemia, and alleviate liver tissue lesions.Conclusion The Suopiyin extract prepared using the optimal process effectively increases the contents of total flavonoids and total polysaccharides and exhibits good antioxidant and hypoglycemic activities both in vitro and in vivo.