Objective This study aims to investigate the characteristics of protein components and the types of cloudy proteins in Tribute citrus turbid juice.Methods Proteomics was used to analyze the proteomic features of the supernatant proteins and precipitated proteins in Tribute citrus turbid juice.Results There were significant differences in the electrophoresis bands of the supernatant proteins and precipitated proteins in Tribute citrus turbid juice. Compared with the supernatant proteins, precipitated proteins contained 2 897 differential proteins, including 2 487 up-regulated differential proteins and 410 down-regulated differential proteins. The up-regulated differential proteins in Tribute citrus turbid juice were mainly enriched in metabolic pathways including tyrosine metabolism, fatty acid degradation, and phagosome, mainly from cellular components such as chloroplasts, cytoplasm, and nucleus. Six types of cloudy proteins were screened from up-regulated differential proteins in Tribute citrus turbid juice, including one pathogenesis-related protein and five glycoproteins.Conclusion Proteomics can be used to explore the protein differences in Tribute citrus turbid juice.