Objective To achieve rapid on-site screening of T-2 toxin and establish an analytical method for the determination of T-2 toxin in corn using aggregation-induced emission (AIE) immunochromatographic test strips.Methods Aggregation-induced emission fluorescent nanoparticles (AIEFNPs) were prepared by the swelling method, and antibodies were coupled via electrostatic adsorption to obtain immune probes. The fluorescent immunochromatographic test strips were constructed by optimizing the experimental parameters.Results The constructed AIE fluorescent immunochromatographic method exhibits a good linear relationship in the range of 0.10~5.00 ng/mL. The regression equation was y=-0.571 41 lg(x)+0.750 88 (R²=0.967 5), with a method detection limit of 0.09 ng/mL. The spiked recovery rates range from 81.82% to 92.46%, and the coefficient of variation is less than 10%.Conclusion This method demonstrates good accuracy and high sensitivity, making it suitable for on-site large-scale screening of T-2 toxin in corn.