Objective To develop the medicinal and food resources of Lonicera japonica, improve the yield of polysaccharides, determine the antioxidant activity of different organs, and reduce the waste of natural Chinese medicine resources.Methods Different organs of L. japonica are utilized as raw materials, with polysaccharide yield as the primary investigation index. The response surface method is used to determine the optimal extraction process. The in vitro antioxidant activity of different organs is assessed and analyzed by metrological methods.Results The optimal extraction conditions are as follows: solid-liquid ratio of 1∶25 （g/mL）, ultrasonic temperature of 80 ℃, ultrasonic time of 60 min, ultrasonic power of 190 W. Under these conditions, the polysaccharide yield is as high as 7.01%. The antioxidant activity results demonstrate that different organs of L. japonica exhibit antioxidant effect, with the strength ranking as: leaves≈flowers>vines>fruits. Cluster analysis shows that L. japonica leaves and flowers are classified into one category, making them preferred resources for extracting L. japonica polysaccharides.Conclusion The ultrasound-assisted method for extracting L. japonica polysaccharides achieves high yield, and the extracts from different organs maintain high antioxidant capacity.