Objective To investigate the effect of exogenous glutathione (GSH) on improving antioxidant capacity in aging mice.Methods Aging mice were administered GSH by gavage at different doses (166.7, 83.3, and 41.7 mg/kg) continuously for 30 days. Levels of malondialdehyde (MDA), 8-isoprostane, protein carbonyl (PCO), and reduced GSH in serum and liver homogenate, as well as activities of total superoxide dismutase (T-SOD) and glutathione peroxidase (GSH-PX), were measured.Results Compared with the blank control group, the high-dose GSH group showed significant reductions in MDA and PCO levels, increased T-SOD activity in serum, and elevated reduced GSH content in aging mice (P<0.05). No adverse effects on body weight gain or organ indices were observed.Conclusion GSH effectively improves oxidative stress status during the aging process.