Ultrasound-assisted enzymatic extraction and structural characterization of pectin from the tender leaves of Abelia macrotera
CSTR:
Author:
Affiliation:

1.School of Food Science and Technology, Hunan Agricultural University, Changsha, Hunan 410128, China;2.Agricultural Product Quality Monitoring Center of Changsha, Changsha, Hunan 410003, China

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    Objective To clarify the physicochemical properties, microscopic structure, monosaccharide composition, relative molecular mass, and structural characteristics of pectin in the tender leaves of Abelia macrotera.Methods Pectin from the tender leaves of Abelia macrotera was extracted by ultrasound-assisted cellulase method. The microscopic structure was observed under scanning electron microscope. The monosaccharide composition was determined by high performance liquid chromatography. The relative molecular mass was determined by polysaccharide hydrolysis derivatization. The possible structure was inferred by infrared spectroscopy.Results The yield of pectin from the tender leaves of Abelia macrotera is 23.38%, the esterification degree was 1.17%, and the galacturonic acid content was 72.16%. The pectin is in the form of flakes with cracks. The monosaccharides mainly consist of galacturonic acid, glucose, arabinose, galactose, rhamnose, mannose and xylose. The weight average molecular weight (Mw) and number average molecular weight (Mn) are 7.069×105 and 3.434×105, respectively, with an Mw/Mn of 2.06. It has typical spectrum characteristics of pectin absorption.Conclusion The pectin extracted from the tender leaves of Abelia macrotera by ultrasound-assisted enzyme method has a low linear structure and is mainly composed of RG-I type structure containing shorter side chains, which meets the commercial requirements.

    Reference
    Related
    Cited by
Get Citation

毛琪琪,罗扬,王姜林,等.双翅六道木嫩叶果胶超声波辅助酶法提取及结构表征[J].食品与机械英文版,2025,41(7):18-23.

Copy
Related Videos

Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:November 18,2024
  • Revised:April 11,2025
  • Adopted:
  • Online: July 12,2025
  • Published:
Article QR Code