Objective In this study, an optimized strategy was proposed to address the limitations of current methods for detecting selenium species in selenium-enriched edible oils, such as the transformation between selenium species, long enzymatic hydrolysis time, and the limited number of detectable selenium species.Methods Protease K-assisted hydrolysis was performed using accelerated solvent extraction, and a high performance liquid chromatography-inductively coupled plasma mass spectrometry (HPLC-ICP-MS) method was established.Results At 40 ℃, with a proteinase K dosage of 35 mg and a static extraction time of 5 min, the extraction rate of selenium species could reach a maximum of (83.65±2.36)%. Eight target selenium species could be effectively separated within 20 min, with good linearity in the range of 2~100 μg/L (R²≥0.999 3), detection limits of 0.20~0.60 μg/kg, quantification limits of 0.60~1.80 μg/kg, spiked recovery rates between 82.3% and 112.3%, and relative standard deviations ≤5.60%.Conclusion This method improves the efficiency and accuracy of selenium speciation analysis.