［Objective ］ To verify the content of sweeteners in ready -made beverages in Hunan province,China,and the potential health risks to adolescents.［Methods ］ Total 850 samples of prepared beverages were collected from beverage stores across Hunan province,and the content of sweeteners in ready -made beverages was detected by high performance liquid chromatography -tandem mass spectrometry (HPLC -MS).Combined with the consumption of ready -made beverages among adolescents,the exposure levels of sodium saccharin,cyclamate and acesulfame in ready -made beverages among adolescents in Hunan province were evaluated.［Results］ The average daily exposure and P 97.5 exposure of ready -made beverage cyclamate in adolescents in Hunan Province were 0.024 8 mg/kg BW (0.230% of ADI ) and 0.157 0 mg/kg BW (1.430% of ADI ),respectively,and the average daily exposure and P 97.5 exposure of acesulfame were 0.017 6 mg/kg BW (0.120% of ADI ) and 0.091 2 mg/kg BW (0.608% of ADI ),respectively.The intake of sweeteners was below the safe intake value.［Conclusion ］ At present,the dietary exposure of freshly prepared beverage sweeteners in Hunan Province poses a low risk to the health of adolescents and is at an acceptable level.