Isolation, identification and control of spoilage microorganisms in Xiangwei stewed bean
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(1. College of Food Science and Chemical, Shaoyang University, Shaoyang, Hunan 422000, China;2. Key Laboratory of Bean Products Processing and Safety Control, Shaoyang, Hunan 42200, China)

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    Abstract:

    Objective: To better control the growth of spoilage microorganisms, and ensure the food safety of Xiangwei Luodou dry. Methods: The putrefactive microorganisms in Xiangwei dried tofu were identified by plate scribing method, colony morphology observation, Gram staining, 16S rDNA and ITS sequence. Results: A total of 9 strains were isolated andscreened. KZ2780-3 and KZ2780-4 are Bacillus subtilis, NZ2559-1 is Bacillus cereus, and JZ2559-5 is Bacillusamylolyticus amyloliquefaciens, FZ2559-2 for Klebsiella pneumoniae, SZ2641-5 for Enterococcus faecium, RZ2641-7 for Staphylococcus haemolyticus, BZ2780-1 for Acinetobacter baui, CZ2780-5 for Penicillium citri. Conclusion: The strains causing dry rot of Xiangwei bean are Bacillus subtilis, Bacillus cereus, Bacillus amyloliquefaciens, Klebsiella pneumoniae, Enterococcus faecium, Staphylococcus hemolyticus, Acinetobacter baui and Penicillium citri. The control methods of Xiangwei stewed bean dry are ultra-high temperature instantaneous sterilization technology, plasma sterilization technology, natural antibacterial substances combined with biological preservatives, vacuum packaging, air conditioning preservation and so on.

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彭 鑫,李冰鑫,马 芳,等.湘味卤豆干中腐败微生物的分离鉴定及控制[J].食品与机械英文版,2024,40(4):172-178.

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History
  • Received:August 19,2023
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  • Online: May 21,2024
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