Objective： Based on transcriptome sequencing analysis, the effect of grape seed procyanidins on HepG2 cell genes and related functions was identified. The key genes and metabolic pathways involved in the treatment of HepG2 cells with procyanidins were confirmed. Methods: Through transcriptome sequencing, screening differential genes, gene functional annotation and enrichment analysis of the KEGG pathway, transcriptome studies were conducted on grape proanthocyanidin-treated cells. Results: After treating HepG2 cells with grape anthocyanins, the main biological processes enriched are cellular processes, metabolic processes, biological regulatory processes, immune system processes, reproductive regulation, and growth regulation. Research has found that 12 key differentially expressed genes in cell apoptosis are associated with TNF, p53, MAPK, PI3K-Akt and NF-κB signaling pathway is closely related. Conclusion: Cell apoptosis is closely related to the TNF signaling pathway, p53 signaling pathway, PI3K/Akt signaling pathway, NF-κB signaling pathway and MAPK signaling pathway.