Amide alcohol drugs have a long half-life and are difficult to excrete through the metabolic process after entering the human body. Excessive amide alcohol drugs will cause serious harm to human organs. Therefore, China has formulated a strict maximum residue limit standard for amide alcohol drugs, in which chloramphenicol can’t be detected in all edible tissues. This review introduces the application of chromatography, mass spectrometry, electrochemical method, electrochemiluminescence method, immunoassay, fluorescence method and surface-enhanced Raman spectroscopy method in the detection of amide alcohol drugs in animal-origin foods, and the advantages and disadvantages of these methods are analyzed and new techniques for the detection of amidol alcohol drugs in the future are prospected.