Objective: Aiming at the problem that strawberry quality was easy to be damaged under impact in processing process. Methods: The relationship among impact energy loss, internal stress and impact damage of strawberry during impact was studied by display dynamics simulation method. The impact of impact parameters on damage degree and sensitivity was analyzed by response surface method. Results: When the strawberry collides with the steel plate at 23 ms, the ratio of strawberry damage volume reached the maximum (7.83%), and at the same time, the impact energy loss (95.60 mJ) and internal stress (0.238 4 MPa) both reached their maximum values. The error of collision simulation results was less than 5.0%, and the simulation method was reliable. Conclusion: The dynamic response of internal stress in strawberry is caused by the change of impact energy, which determines the impact damage degree. The influencing factors of fruit damage sensitivity were: contact material > impact velocity > impact angle.