Objective: This study aimed to establish a method for the simultaneous determination of 80 veterinary drugs including quinolones, sulfonamides, receptor agonists, glucocorticoids, macrolides, tetracyclines, antiviral drugs in chicken by QuEChERS - ultra high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS）. Methods: The samples were extracted by 1% formic acid acetonitrile aqueous solution, purified by QuEChERS multi-veterinary drug residue purification column, detected by ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS） multi-reaction monitoring mode, and quantified by matrix standard curve external standard method. Results: The results showed that 80 kinds of veterinary drugs were highly responsive under specific mass spectrometry conditions, and the samples were sensitive to detection under the conditions of mass spectrometry without interference of foreign peaks. The correlation coefficient of 80 drugs were greater than 0.99 in the range of 5.0～100 μg/L, with good linearity. The detection limit of 80 veterinary drugs were 1.0 μg/kg. The average recovery of spiked concentration of 2.0, 5.0, 10.0 μg/kg in the sample was 62.2%～106.8%; RSD was 2.07%～10.85%. Conclusion: The recovery rate of 80 drugs in the samples conformed to the standard requirements of GB/T 27404—2008 Code for Laboratory Quality Control - Physical and Chemical Testing of Food. The method was suitable for the screening and determination of 80 veterinary drug residues in chicken.