Objective: A gas chromatography-tandem mass spectrometry method was established for the simultaneous determination of 24 pesticide residues in fresh edible fungi. Methods: Samples were homogenized and then shaken horizontally for extraction with water, acetonitrile and salt. After high-speed centrifugation, the supernatant was removed and concentrated with nitrogen then reconstituted with ethyl acetate and finally determined by GC-MS/MS. Electron impact ionization （EI） was used for ionization, multiple reaction monitoring （MRM） mode was used to collect target fragments by multiple reaction monitoring （MRM） mode and internal standard method was used for quantitative analysis. Results: 24 pesticides showed good linearities in their respective concentration ranges with correlation coefficient （R²） that were both greater than 0.998 6. The limit of detection （LOD） and quantification （LOQ） of the method were in the ranges of 2.0~3.0 μg/kg and 5.0~10.0 μg/kg, respectively. Average recovery rates of 24 pesticides ranged from 76.4% to 101% with the relative standard deviation （RSD） being 1.79% to 8.98%. Conclusion: The method was simple to operate, rapid, good recovery, high sensitivity and good reproducibility and suitable for the determination of 24 pesticide residues in fresh edible fungi.