Cloud point extraction coupled with Isooctane back extraction for determination of eight pesticide residues in tea beverages by gas chromatography mass spectrometry
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(1. Wuxi Center for Disease Control and Prevention, Wuxi, Jiangsu 214023, China; 2. Nanjing Medical University, Nanjing, Jiangsu 211166, China)

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    Abstract:

    Objective: Rapid determination of organochlorine and pyrethroid pesticide residues in tea beverages. Methods: Selecting the non-ionic surfactant PEG-4000 as cloud point extraction, pesticides were extracted with isooctane by ultrasonic back extraction. The upper layer of isoctane solution was analyzed by Gas Chromatography/Mass. The isooctane solution was separated by HP-5 ms UI capillary column and analyzed qualitatively and quantitatively by GC/MS. Results: The eight pesticides showed a good linear relationship between 0.01~2.0 and 0.02~4.0 mg/L, respectively, with the correlation coefficients (R) of over 0.995. The detection limit and quantification limit of this method were 0.003~0.008 mg/L and 0.01~0.02 mg/L, respectively. The average recovery was 78.3%~98.4%, RSD was 2.7%~8.5% (n=6). Conclusion: The method is simple in operation, high in enrichment rate, practical, safe and economical, which is suitable for rapid determination of pesticide residues in tea drinks.

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谢 洁,周 闰,孔令灿,等.浊点萃取—异辛烷反萃取—气相色谱质谱法测定茶饮料中8种农药残留[J].食品与机械英文版,2023,39(1):31-36,156.

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  • Received:October 10,2022
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  • Online: April 25,2023
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