Objective: This study aimed to eslablish a method for the determination of 15 benzodiazepine sedatives in aquatic products by liquid chromatography tandem mass spectrometry (HPLC-MS/MS). Methods: The sample was extracted with acetonitrile solvent, and the extract was purified by solid phase extraction (Oasis PRIME HLB) column. After concentration, the mobile phase was used to determine the volume. The mobile phase consisted of 1% formic acid aqueous solution and acetonitrile solvent. The method was performed in multiple reaction monitoring (MRM) mode and quantified by internal standard method. Results: The results showed that the linear range of 15 sedatives was 1~100 ng/mL (R＞0.99). The limit of detection (LOD) was 0.1~0.3 μg/kg and the limit of quantification (LOQ) was 0.3~1.0 μg/kg. The average recoveries were 78%~125% and the relative standard deviations (RSD) were 1.52%~12.02%. Conclusion: The method has the advantages of simple pretreatment, good purification effect and accurate results. It is suitable for the rapid detection of 15 kinds of sedatives in aquatic products.