Objective: The study aimed to explore the effects of composite probiotics on the intestinal regulation. Methods: By constructing mouse model of constipation, intestinal damage and diarrhea, the intestinal ink propulsion, defecation and intestinal mucosal tissue sections in the control group, model group and probiotics-intervention group were evaluated. The diarrhea index and intestinal mucosal damage index were also considered for comprehensively assessment. Results: Compared with the model group, the mid-and high-concentration of composite probiotics could significantly improve the defecation amount and weight, and the small intestinal ink propulsion rate. Moreover, the intestinal mucosal damage score and histopathological damage were apparently reduced in the probiotics-intervention group at mid-and high-concentrations. The intestinal mucosal damage of diarrhea model mice was relieved by the composite probiotics at all tested concentrations. The mid-and high-concentration of the composite probiotics helped reduce constipation, diarrhea, and intestinal mucosal damage, and the higher concentration worked more effectively. Conclusion: The composite probiotics can be used as an alternative for the probiotics market and provide references for the development of probiotics product.