Objective: To investigate the effect of moisture content of heated cigarette on releases of aerosol main components. Methods: Three different types of heated cigarettes were used to study the effects of moisture content of cigarette sticks on the release of main aerosol components by gas chromatography. Results: ① The equilibrium humidity was a positive correlation between the moisture contents of tobacco materials and releases of total aerosol particulate matters. ② The moisture contents of heated cigarettes stored at different equilibrium humilities had a great effect on releases of glycerol and nicotine in aerosol, but a small effect on release of propylene glycol. ③ The release of nicotine in aerosols increased first and then decreased with the increase of equilibrium humidity, and release of nicotine in aerosol was the highest after heated cigarette stored at 50% relative humidity. Conclusion: The water content of three kinds of heated cigarette tobacco materials has influence on the release amount of total aerosol particles, smoke agent and nicotine.