Objective:The purpose of this study was to explore the antioxidative activity in vitro experiments and the anti-fatigue activity of chickpea oligopeptides in mice. Methods:In this study, the antioxidant capacity of chickpea oligopeptide was detected by in vitro method, and the anti-fatigue capacity of chickpea oligopeptide was comprehensively evaluated by detecting physiological and biochemical indexes of mice. Results:The superoxide anion radical scavenging ability IC50of chickpea oligopeptide was 4.26 mg/mL, which was significantly higher than that of GSH positive control group (P＜0.05); The reducing power of chickpea oligopeptide was significantly higher than that of GSH positive control (P＜0.05). In addition, at low, medium and high doses, the fatigue tolerance, muscle glycogen and liver glycogen reserve capacity of mice were improved, and the concentration of serum urea nitrogen, malondialdehyde and lactic acid was decreased (P＜0.05). It can significantly increase the activities of catalase, superoxide dismutase and glutathione peroxidase (P＜0.05). Conclusion:Chickpea oligopeptides have significant anti-fatigue effects.