Objective:To explore the relationship between functional properties of rice glutelin fibril aggregates (RGFAs) solution formed by different storage and heating time.Methods:The RGFAs samples were prepared by heating rice glutelin (RG) at 85 ℃ and pH 2.0 for 2~15 h, and stored at 4 ℃ for 1 day and 4 days, respectively. The surface hydrophobicity, emulsifying property, shear viscosity of RGFAs samples as well as the gelatinization characteristics and hydrolysis rate of the mixture of RGFAs and starch were measured.Results:After storage at 4 ℃ for 4 days, the emulsifying properties were significantly improved. The surface hydrophobicity of RGFAs solution formed by heat treatment for 2~10 h decreased significantly (P＜0.05), while the surface hydrophobicity of RGFAs solution formed after 15 h of heat treatment did not change significantly (P＞0.05), and the shear viscosity of 15 h-RGFAs solution decreased. The gelatinization temperature of the mixture of starch and 2 h-RGFAs stored for 1 day was the lowest (83.98±0.08) ℃, and the hydrolysis rate of the mixture of starch and RGFAs samples stored for 1 day was lower than that of other samples.Conclusion:The inhibition of wheat starch hydrolysis by RGFAs samples stored for 1 day was better than that by RGFAs samples stored for 4 days.