Objective: This study focuses on exploring the effect of perilla leaf extract on hypoglycemic in type 1 diabetic mice. Methods: Streptozotocin （STZ） was used to induce the establishment of type 1 diabetes （T1DM） mouse model, and then the normal group, model group, perilla leaf extract high-dose group （400 mg/kg）, perilla leaf extract low-dose group （200 mg/kg）, positive group （150 mg/kg） were investigated. During the test, the food intake and water consumption of the mice were recorded, and the fasting blood glucose （FBG） of T1DM mice was measured every week, and the glucose tolerance level （OGTT） was measured on the 28th day of gavage. Blood is taken from the eyeball later, the heart, liver, spleen, lung, and kidney are taken after dissection to determine the organ coefficient. Determination of total cholesterol （TC）, triglycerides （TG）, glycosylated hemoglobin （GHb）, superoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA） related indicators. Results: The results showed that compared with the model group, the food intake and water intake of T1DM mice treated with perilla leaf extract decreased, food titers increased significantly （P＜0.05）, and the FBG of T1DM mice treated with perilla leaf extract decreased significantly （P＜0.05）, the levels of TC, TG, and MDA were also significantly reduced （P＜0.05）, SOD, CAT significantly increased （P＜0.05）. Conclusion: The perilla leaf extract can hypoglycemic in type 1 diabetic mice.