Solid-phase extraction and accurate analysis on food additives in marinated meat products
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(1. Department of Food and Biological Engineering, Yantai University of Technology, Yantai, Shandong 264005, China; 2. School of Agricultural Engineering and Food Science, Shandong University of Technology, Zibo, Shandong 255000, China)

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    Abstract:

    Objective: To establish an accurate method for the determination of benzoic acid, sorbic acid and saccharin sodium in soy sauce brine meat products. Methods: The samples were extracted by ethyl acetate, SPE-MCX solid phase extraction, and detected by HPLC simultaneously. Results: The determination was performed on Agilent Zorbax Eclipse XDB-C18 column (250 mm×4.6 mm, 5 μm) with mobile phase consisting of 75% 0.02 mol/L ammonium acetate solution and 25% acetonitrile. The column temperature was set at 25 ℃, and the flow rate was 0.8 mL/min. The sample size was 10 μL. The detection wavelength was 230 nm. In the range of 0.5 ~ 10.0 mg/L, the peak area of the three additives showed a good linear relationship with the mass concentration (R2 > 0.990). The detection limits of the three additives were all lower than 3.52×10-2 mg/kg, the recoveries were 95.44% ~ 101.24%, and the relative standard deviations were all less than 2.92%. Conclusion: The method is simple, sensitive, rapid and accurate. It is suitable for the routine detection of benzoic acid, sorbic acid and saccharin sodium in soy sauce brine meat products.

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王晓君,李凤玲.酱卤肉制品中食品添加剂的固相提取与精确分析[J].食品与机械英文版,2021,37(8):94-99.

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History
  • Received:April 27,2021
  • Revised:
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  • Online: February 15,2023
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