Objective: To establish a dual real-time PCR method for the detection of orange and citrus components in orange juices. Methods: Through the design and screening of primers and probes for orange and citrus components. Through the detection of fruits, found the rule of fluorescence amplification of orange and citrus. Using the ratio difference of orange and citrus in FAM/VIC two fluorescence channels to determine the composition of orange and citrus. The lowest doping ratio detected by the method was verified by the experiment of orange juice adulteration, tested the commercial orange juice and verify the feasibility of the method. Results: There were fluorescence amplification curves in both FAM and Vic channels in pure orange fruits, and the fluorescence ratio of FAM/VIC channel was between 0.5±0.2, while only FAM channel had strong fluorescence amplification curve in pure citrus fruits. In the simulation experiment, it could be detected that there are more than 10% citrus juice in orange juice. Conclusion: The results showed that this method can be used to detect the orange and citrus components in commercial orange juices, and can be used to identify the fake orange juices.