In order to investigate the antioxidant and antibacterial activity of different polar parts (petroleum ether part, ethyl acetate part, n-butanol part and water part) from Manilkara zapota leaves. The DPPH radical-scavenging assay, ABTS radical-scavenging assay and FRAP antioxidant capacity assay were carried out. And the antibacterial effects of different polar parts from Manilkara zapota leaves against Bacilus subtillis, Escherichia coli and Staphylococcus aureus were studied. The results showed that the extracts obtained from ethyl acetate extraction had the highest antioxidant activities; the IC₅₀ of the DPPH radical-scavenging and ABTS radical-scavenging were (0.019±0.010) mg/mL, and the Trolox Equivalent Antioxidant Capacity (TEAC) value was (5.824±0.234) mmol/g. The ethyl acetate-extracts, at the concentration of 0.50 mg/mL, had >50% scavenging rate according to the ABTS radical-scavenging assay. The ethyl acetate-extracts also showed outstanding resistant activity against Staphylococcus aureus, the MIC and MBC were 0.313 mg/mL and 0.625 mg/mL, respectively. It was confirmed that the ethyl acetate-extracts from Manilkara zapota leaves showed outstanding antioxidant activity and antibacterial activity, and they could be used in the exploring of natural antioxidants and antibacterial agents.