A total of 8 strains of bacteria were isolated from 6 pickle samples in Northeast, Shandong, Guizhou, Sichuan and other places. Using simulated gastric acid experiments， morphological observations, physiological and biochemical experiments, lactic acid bacteria qualitative experiments, two strains of lactic acid bacteria with high survival rate under pH 2.5 conditions were obtained by simulation experiment. After 16S rDNA sequencing, it was determined that the two acid-resistant lactic acid bacteria were Lactobacillus brevis isolated from Shandong sugar garlic pickles numbered Shandong SRB and Lactobacillus casei isolated from Northeast sauerkrautnumbered Northeast HS.