Taking the cultivable bacteria in the air of high-temperature Daqu fermentation room as the research objects to study the characteristics of separation, identification and enzyme production. The cultivable bacteria were used to take liquid state fermentation in sorghum mash, and the metabolites were analyzed by gas chromatography-mass spectrometry. Results: 8 strains were isolated from the air in the high-temperature Daqu fermentation room, and they were: Bacillus amyloliquefaciens, Bacillus subtilis, Bacillus cereus, Pedobacter suwonensis, Sphingomonas sanguinis, Massilia sp., Delftia sp. and Sphingobacterium sp.. After the enzyme production tests, three dominant functional bacteria, Bacillus subtilis, Bacillus amyloliquefaciens, and Bacillus cereus, which all had the capacity of decomposition for cellulose , starch and proteins. The growth curve of 3 strains showed that liquid culture reached logarithmic growth phase for 4 h. After 48 hours of fermentation in the sorghum mash medium, the dominant metabolite was mainly 3-hydroxy-2-butanone, and the content of tetramethylpyrazine was also high, and it metabolizes to produce a small amount of higher alcohols, phenols, esters and other aromatic substances. It hopes to provide a theoretical basis for studying the correlation between Daqu bacteria and air bacteria.