In order to simultaneously determine narirutin, naringin, hesperidin, neohesperidin and limonin in pomelo, the effects of elution conditions and measurement wavelength of high-pressure liquid chromatography on the determination results were studied. The results showed that the optimal measurement conditions were as follows. Chromatographic column of Bio-Bond 5 μm C₁₈ (4.6 mm × 250 mm was used, and the injection volume was 10 μL; the detection wavelengths of flavonoid glycosides and limonin were 283 nm and 220 nm, respectively; The phase: included acetonitrile (A) and water (B) with gradient elution of 0 min, 22% A, 8 min, 22% A, 10 min, 95% A, 16 min, 95% A, 18 min, 22% A, and 20 min, 22% A, with 1 mL/min flow rate. Under the control o these conditions, the above five components were well separated; the correlation coefficient of the standard curve was greater than 0.999 7, and the detection limit was 0.69~1.72 mg/L. The quantification limit was 1.51~3.15 mg/L, and the relative standard deviation of repeatability was less than 2%. The sample recovery rates were all greater than 98%, and the relative standard deviation of the sample recovery rates were all less than 2%.