Aiming at the problem of fresh e-commerce food safety, based on the existing supervision system, we constructed a fresh e-commerce food quality and safety supervision simulation model composed of fresh e-commerce suppliers, fresh e-commerce platform, government regulatory agencies and consumers. We evaluated the quality preference and investment strategies of the suppliers, and the effects of fresh food safety brought by government and regulatory policy for platform were also investigated. The results showed that that the effective government sampling inspection on the platform could improve the safety of the fresh e-commerce products, and the proportion of sampling inspection on the fresh e-commerce platform was conducive. Increasing the punishment intensity of the platform for suppliers was more effective than increasing that from the government.