The toxicology of licorice oil was researched through the acute toxicity test of mice, genotoxicity test of mouse sperm deformity and subacute toxicity test, and made a safety evaluation on it. The experimental results showed that the acute oral LD₅₀ of licorice oil on mice was greater than 15.0 g/kg·BW, which was classified as non-toxic. The mouse sperm deformity genetic toxicity test was negative, indicating that the test substance had no mutagenic effect. No abnormal changes in animal health status, biochemistry, hematology indexes and organ tissue morphology were observed in the 30-day feeding experiment. Based on this, it was estimated that the maximum non-effective dose of the product was greater than 5.0 g/kg·BW, which was 150 times as high as the recommended human intake. Therefore, licorice oil had no acute toxicity, subacute toxicity and genotoxicity, and licorice oil was excellent edible oil with higher safety.