An UHPLC-MS/MS method for rapid analysis of tea for the potential presence of illegally added flavorings
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    Abstract:

    A qualitative and quantitative method for simultaneous detection of 7 potential presence of illegally-added flavorings, including trans-anethole, coumarin, estragole, lawsone, methyleugenol, pulegone and trans-cinnamaldehyde in tea samples. Tea samples were extracted with acetonitrile-water served as a mixed solvent, subjected to whirlpool oscillation and then diluted properly. After that, anhydrous sodium sulfate, primary secondary amine (PSA) and octadecylsiyl silica gel (C18) were added to the solvent for purification. The centrifugation, the supernatant was filtered over a membrane and separated on a Kinetex C18 column. These 7 target compounds were determined by UHPLC-MS/MS in MRM mode, and quantified utilizing a matrix-matched external standard method. The results showed a good linearity from 0.8~1 600.0 μg/L and the value of the corresponding correlation coefficients (R2) were all larger than 0.999. The values of limit of detection were 0.02~0.03 mg/kg, and those of limit of quantification were 0.05~0.10 mg/kg, the average recoveries were 82.5%~102.5%, and the intra-day and inter-day RSD values were below 16% and 18%, respectively. A survey of 100 commercial tea samples was conducted, showing that trans-anethole, coumarin and estragole were present in green and black tea with maximum concentrations of 65.0, 2.0 and 72.2 mg/kg, respectively.

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梁志森,陈玉珍,周朗君.超高效液相色谱—串联质谱法快速同时检测茶叶中7种香料[J].食品与机械英文版,2020,(11):51-57.

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  • Online: February 18,2023
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