It was investigated the alleviating effect of water-extracts from Moringa oleifera seeds (MWE) on alcohol damage, and explored the effects of Moringa seeds on the physiological changes of drunk nematodes. Different concentrations of MWE were used to interfere with nematodes drunkenness. Its movement, tissue ethanol concentration, calcineurin and acetylcholinesterase activity were evaluated. The results showed that the intervention concentration of MWE was 10 mg/mL, and the movement trajectory, frequency of head swinging and swallowing frequency of the nematode were significantly improved, compared with the control group. However, no significant difference was found compared to the un-treated group. MWE reduced the tissue ethanol concentration and the overexpression of calcineurin, increased the activity of acetylcholinesterase, and the resistance against alcohol stress in nematodes. These results suggest that the low concentration of MWE can protect alcohol-induced movement disorder in Caenorhabditis elegans.