To study the effect of short-term intake of high edible oil on the viscera coefficient and gastrointestinal motility in mice and providing the basis for rational dietary oil, 10 SPF KM mice were randomly divided into the control group and the edible oil group. The mice in the edible oil group were given edible oil twice a day, 0.3 mL each time, according to 20 mL/(kg·d) and mace in the control group were given equal amounts of distilled water for 13 days. The viscera coefficient and gastrointestinal motility of mice were compared. It was found that the dietary amount of edible oil group decreased and the amount of drinking water increased. Compared with the control group, edible oil reduced the coefficients of liver, spleen and thymus in mice, but the difference was not statistically significant (P＞0.05). In terms of gastrointestinal motility, edible oil could increase the small intestine propulsion rate of mice and slow down the gastric emptying rate of mice, with no statistically significant difference compared with the control group (P＞0.05). The content of serum D-xylose in the control group was higher than that in the edible oil group, but the difference was still not statistically significant (P＞0.05). The study indicated that short-term high intake of edible oil had little effect on viscera coefficient and gastrointestinal motility in mice.