The bee pollen of oilseed rape was used to investigate the effect of enzymatically breaking the wall for protein extraction, and the optimal conditions of the extraction were determined by single factor test and response surface analysis. After mixing 2.5 g of peak pollen and 50 mL of pH 5.96 aqueous solution, 0.4% volume fraction compound plant invertase (100 FBG/g) was added and then stirring and extracting at 42 ℃ for 6.5 h. Under the control of these optimal conditions, the protein pollen extracts were separated by Sephadex G-100 gel column to obtain a total of 4 components. However, only one of them was determined to contain protein with 66.77 g/100 g, with the contain of hydrophobic amino acid of 27.52 g/100 g. The amino acid composition analysis and infrared spectroscopy results showed that aromatic amino acids were found in bee pollen protein. Moreover, the circular dichroism chromatography results showed that the proteins in bee pollen were easily digested enzymatically for producing peptides.