To investigate the effect of vacuum freeze drying on phenolic content and in vitro antioxidant activity of four varieties of Chrysanthemum, including Dendranthemamorifolium(Ramat.) Tzvel., Florists Chrysanthemum, Bellis perennis L., and Chrysanthemum morifolium 'Hangju'. The results showed that the total phenolic content of Chrysanthemum was significantly increased after vacuum freeze drying. Among the four varieties, B. perennis L. displayed the highest percentage increase in total phenolics after vacuum-freeze drying (Increaseed by 2.525 times). The DPPH· scavenging capacity, ferric reducing antioxidant property and ORAC were significantly changed except ABTS+· scavenging capacity, which indicated that vacuum-freeze drying has great influence on the antioxidant activity of Chrysanthemum phenolics.