To investigate the bioactive compounds and antioxidant activity of vine, leaves and fruits of the Ficus tikoua Bur. in Guizhou Province, the phenolic compounds, vitamin C, total phenols, total flavonoids were analyzed. Antioxidant activity was evaluated by total reducing power assay (TRPA), free radical scavenging capacity of ABTS⁺· (ABTS) and ferric ion reducing antioxidant power(FRAP) method in vitro, and Pearson correlation analysis was conducted. The results indicated that the contents of bioactive compounds and antioxidant activity were significantly different between the vine, leaves and fruits (P＜0.05). The content of total phenols and flavonoids in vine was significantly higher than that in leaves and fruits (P＜0.05), while the content of VC in leaves was significantly higher than that in fruits and leaves (P＜0.05). Gallic acid, ellagic acid, chlorogenic acid and rutin were the major phenolic compounds in the Ficus tikoua Bur. Phenolic compounds in leaves was significantly higher than that in fruits and vine (P＜0.05). The FRAP of fruit was significantly higher than that of ABTS and TRPA (P＜0.05). The ABTS and FRAP of vine and leaf were significantly higher than that of TRPA (P＜0.05), ABTS and FRAP of fruit were significantly higher than those of vine and leaf (P＜0.05). The antioxidant activity of the samples increased positively with the concentration. Relevance analysis found that total phenols has a strongest contribution to the TRPA value, and the ellagic acid and chlorogenic acid contribute significantly to the FRAP and ABTS, respectively. The Ficus tikoua Bur. can be fully exploited and utilized according to the content of bioactive compounds and antioxidant activity.