Screening and characteristics of heat-resistant strain from concentrated carrot juice
CSTR:
Author:
Affiliation:

Clc Number:

Fund Project:

  • Article
  • |
  • Figures
  • |
  • Metrics
  • |
  • Reference
  • |
  • Related
  • |
  • Cited by
  • |
  • Materials
  • |
  • Comments
    Abstract:

    The heat-resistant strain was isolated and identified by morphological features and ITS rDNA gene sequence analysis. Some physiological and biochemical characteristics of the strain were examined by carbon source utilization experiment. The thermal stability and acid-base stability of the strain were examined by heat and acid-base treatment. With the ultrasonic technology, the polysaccharides, reducing sugar and pigment in cells were examined. The results indicated that the strain SR-1 belongs to Sporobolomyces carnicolor. It was heat-resistant, and grew stably after heat treatment at 80 ℃ for 5~30 min, and at 90 ℃ for 15 min. However, it couldn't grow after treated at 90 ℃ for 20 min. The strain could grow well at pH 6.0~8.0, but could survive under pH 3.0. Hydrochloric acid with 200, 400, 600 and 800 W ultrasound were used to break the cell wall, the supernatant contained 6.02~7.86 mg/mL of reducing sugar, 32.93~41.78 mg/mL of polysaccharide, and the absorption peaks of mycelium pigments were found at waves of 414, 539 and 634 nm.

    Reference
    Related
    Cited by
Get Citation

刘彩琴,魏兴虎,王楠,等.胡萝卜浓缩汁中耐热菌的分离及特性研究[J].食品与机械英文版,2020,(2):38-41,118.

Copy
Related Videos

Share
Article Metrics
  • Abstract:
  • PDF:
  • HTML:
  • Cited by:
History
  • Received:
  • Revised:
  • Adopted:
  • Online: February 16,2023
  • Published:
Article QR Code