The mice in the treatment group were administrated with the BEP at doses of 200, 400, and 800 mg/(kg BW·d) for 30 consecutive days. The liver injury model was established by intraperitoneal injection of CCl₄. The alanine transaminase (ALT), aspartate transaminase (AST) contents in serum, and superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-Px) and glutathione (GSH) levels in liver were measured. Meanwhile, a hepatic pathological examination was conducted. The results of this study showed that Boletus edulis polysaccharides could, to some extent, protect mice from CCl₄-induced liver injury. In addition, Boletus edulis polysaccharides significantly prevent the increase in liver index spleen index, serum ALT, AST, and liver MDA levels, caused by CCl₄ in mice. Boletus edulis polysaccharides could also markedly increase the activities of liver SOD, GSH-Px, GSH. Among the tested doses, 800 mg/(kg BW·d) showed the best effect. Histopathological examinations further confirmed these results.