An analytical method was developed for the determination of 8 kinds of vitamin E isomer (tocopherol and tocotrienol) in vegetable oil by solvent dilution extraction-ultra performance liquid chromatography. The sample was extracted with acetone. The separation was achieved by using PFP column and an elution with the mobile phases of methanol and water (937) at a flow rate of 0.4 mL/min. The identification and quantitative analysis were carried out with fluorescence detector at an ecxitation wavelength of 295 nm and an emission wavelength of 330 nm. The recoveries ranged from 90.0% to 100%, with the relative standard deviation (RSDs) between 0.28% and 1.22%. The linear range was 0.4～40.0 μg/mL and the correlation coefficient (r) was greater than 0.999. The limits of detection were 4.0 mg/kg for each vitamin E isomer. The method was enough for the analysis of vitamin E in actual samples with wide linear range and high sensitivity.