The antibacterial liquid was prepared by dissolving potassium sorbate, sodium dehydroacetate and sodium benzoate in polyvinyl alcohol solution, and then the antibacterial liquid was applied to kraft paper to prepare antibacterial paper for packaging. The inhibitory effects of the antibacterial paper on Escherichia coli and Staphylococcus aureus were tested by the bacteriostatic circle method, and the effects of the coated antibacterial solution on the tensile strength, tear strength, water vapor permeability coefficient, oxygen permeability and microstructure of the antibacterial paper were studied as well. The results showed that the tensile strength, tear strength, water vapor permeability coefficient and oxygen permeability of the antibacterial paper were improved, and the microstructure was more compact and uniform, with good antibacterial properties. The bacteriostatic effect increased with the increase of the concentration of the antibacterial agent, and could be used for packaging of foods.