The Y₆ strain screened from the grape surface was subjected to atmospheric pressure room temperature plasma (ARTP) mutagenesis, and the Saccharomyces cerevisiae strain with strong alcohol production ability, high acid production ability and stable fermentation performance were selected. The single-factor test confirmed that the optimal mutagenesis condition was lethality rate at 98.70% treated for 100 s. The target strain Y₆-8 producing alcohol and producing acid was screened by 2,3,5-triphenyltetrazolium chloride (TTC) method, bromocresol green medium method and Du's tubule, and then its genetic stability was studied. The results showed that the mutagenized strain Y₆-8 was added to the grape juice with the same sugar content, and the alcohol production and acid production capacity of the mutagenized strain were increased by 28.13% and 214.93%, respectively, and the genetic performance was stable.