The effect of hydrolysate from Corbicula fluminea soft tissue on the activation of alcohol dehydrogenase (ADH) in vitro was determined, and its effects of anti-alcohol and liver-protection on mice were also observed by measuring the drunk time, sober-up time, concentration of ethanol in serum, and the contents of malondialdehyde (MDA), triglyceride (TG) and glutathione (GSH) in liver tissue of mice. The results showed that hydrolysate from soft tissue of Corbicula fluminea could activate ADH in vitro with activation rate of 52%. It could also prolong the drunk time and shorten the sober-up time of drunken mice significantly, by 69.42% and 13.40%, respectively, with the comparation of the negative group. It reduced the concentration of ethanol in mice serum significantly (P<0.05) and the contents of MDA and TG in liver tissue of mice significantly (P<0.05), and slowed down the consumption of GSH in mice significantly (P<0.05). Therefore, the hydrolysate from Corbicula fluminea soft tissue could activate ADH in vitro, with the function of anti-alcohol and protecting liver in drunk mice.