In order to objectively and accurately evaluate the uniformity of cut tobacco, reconstituted tobacco and cut stem in tobacco formula, this paper established a method for evaluating the uniformity of tobacco formula based on the difference between the theoretical value and the actual value of total sugar/cellulose. This method was evaluated, applied and compared by constructing different proportion of reconstituted tobacco and cut stem. The results showed that:The content of total sugar and cellulose in cut tobacco, reconstituted tobacco and cut stem is different. Total sugar: cut tobacco ＞ cut stem ＞ reconstituted tobacco, cellulose: reconstituted tobacco ＞ cut stem ＞ cut tobacco. In the experimental range, the use of total sugar and cellulose as indicators for uniformity evaluation has good sensitivity and accuracy. When setting the ratio of reconstituted tobacco and cut stems to 5% to 15%, The sensitivity of cellulose/total sugar and total sugar/cellulose is 32% and 40%. When the blending ratio of actual reconstituted tobacco and cut silk is 6% to 9%, the relative difference between the actual detection value and theoretical prediction of total sugar and cellulose is less than <1%. By evaluated the uniformity of the tobacco formula in the silk-making process and compared with the standard method YC/T 426—2012, our method has good practicability and can be used as a supplementary method to determine the uniformity of the cut tobacco in actual production.