Neonicotine-pyrethroid mixed pesticides are a new type of compound insecticides which have higher activities, broader insecticidal spectrum and longer duration compared with the single-does of λ-cyhalothrin and imidacloprid. They can effectively reduce pesticide residues in agricultural products and lower the harm to human health. Residue analysis methods of neonicotine and pyrethroid pesticides on fruits and vegetables have been studied, but because of the difference of physical and chemical properties of the two pesticides, the detection methods are different. Therefore, it is necessary to establish a rapid screening method for residues of nicotine-pyrethroid pesticides. In this study, imidacloprid and λ-cyhalothrin were taken as representatives to study residues and detection technology of neonicotine-pyrethroid mixed pesticides in fruits and vegetables. Moreover, the application of imidacloprid and λ-cyhalothrin by using QuEChERS-GC/LC-MS was emphasized.