The object of this study was to in vitro evaluate the effect of extracellular products of P. fluorescens, A. hydrophila and A. sobria on the growth and metabolism of S. putrefaciens at low temperature. The extracellular products of these three strains were mixed with sterilized brain heart infusion broth (BHI), and S. putrefaciens were inoculated into the mixed culture, and the quality indicators including microbiological, extracellular protease, biofilm, TVB-N, amino acid and putrescine changed during 12 days at 4 ℃. The results showed that extracellular products of P. fluorescens, A. hydrophila and A. sobria had little effect on the lag phase and exponential phase of S. putrefaciens, but extracellular products of A. sobria could inhibit the growth of S. putrefaciens during the stationary phase. In addition, compared to the extracellular products of P. fluorescens and A. hydrophila, the extracellular products of A. sobria had the strongest inhibitory effect on the biofilm and extracellular protease formation and spoilage potential of S. putrefaciens.