Taking β-cyclodextrin as the wall material, embedding rate as the detection index, and single factor test results as the basis for orthogonal test, determined the optimal inclusion process of Cinnamomum jensenianum leaves volatile oil. Meanwhile, the inclusion compound was evaluated using thin-layer chromatography (TLC), ultraviolet spectroscopy (UV), differential scanning calorimetry (DSC) and micrography. The experiment results indicated that, the optimal preparation process conditions for the inclusion compound of Cinnamomum jensenianum volatile oil were as followed: ultrasonication time of 8 min, mass ratio of core material to wall material of 1︰8, ultrasonic wave power of 150 W, mass ratio of β-cyclodextrin to water of 1︰9, and ultrasonic temperature of 40 ℃. After characterization analysis through TLC, UV, DSC and micrography, the results suggested that the volatile oil had formed the inclusion compound with β-cyclodextrin. Under such conditions, the inclusion compound was stable in property, with Uniform particle size, and favorable powder morphology.