Taking glycyrrhizic acid extraction rate was used as an index, the technological conditions of extraction of glycyrrhizic acid from Licorice wad optimized by two-stage method of combined enzymes and ultrasonic. When the mass of glycyrrhizic acid was 1 g, the optimum technological conditions for the enzymatic stage were as follows: the optimal ratio of combined enzymes (pectinase∶cellulose) was 1∶3 (U/U); the dosage of combined enzymes was 175 U/g·Substrate, reacted at pH 5.5 for 1 h at 50 ℃; and the particle size of raw material was 40 mesh with the ratio of material to liquid 1∶25 （g/mL）. The optimum technological conditions for the ultrasonic stage were as follows: the ultrasonic extraction solvent is 20% ethanol, and the ratio of material to liquid is 1∶35 （g/mL）, ultrasonic treating for 15 min at 40 ℃ with 350 W. Under these optimum conditions, the extraction rate of glycyrrhizic acid could reach（90.44±0.14）%.