Optimization on extraction technology and analysis of subunit component of bamboo bean albumin
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(1. School of Biotechnology and Food Engineering, Changshu Institute of Technology, Changshu, Jiangsu 215500, China; 2. School of Food Science and Engineering, Jilin Agriculture University, Changchun, Jilin 130118, China)

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    Abstract:

    The osbrone classification method was used to extract bamboo bean albumin, the response surface method was designed to optimize the extraction technology, and its subunit component was analyzed. Design-Expert 8.05b software was used to establish mathematical models for the extraction process of bamboo bean albumin. The best extraction technology was obtained: stirring rate: 356 r/min, extraction temperature: 44 ℃, extraction time: 1.5 h, ratio of material to liquid: 111.20 (g/mL). Under this condition, the extraction rate is 27.56% (n=3), which had no obvious difference with the predicted value of 27.25%. In this case, the purity of bamboo bean albumin was (86.50±2.17)%. The analysis of SDS-PAGE gel electrophoresis showed that the main subunit molecular weight of bamboo bean albumin was 49.6 kDa and 27.6 kDa.

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姚海霞,王娟,王立梅,等.竹豆清蛋白提取工艺优化及亚基组成分析[J].食品与机械英文版,2018,34(6):174-179.

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History
  • Received:January 31,2018
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  • Online: March 17,2023
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