In order to further develop the resources of Podocarpus Nagi, the technology of ultrasonic assisted soxhlet extraction for the extraction of Podocarpus nagi was optimized by single factor and response surface tests. The physicochemical properties and fatty acid composition were analyzed. The results showed that the optimum extraction conditions were as follows: the extraction temperature 75 ℃, the solid-liquid ratio 140 （g/mL）, the extraction time 150 min and extraction solvent n-hexane. Under this condition, the yield of bamboo nut oil was 29.32%. The analysis via GC-MS method showed that nine kinds of fatty acids were detected in the bamboo nut oil, including 95.81% unsaturated fatty acids and 76.27% polyunsaturated fatty acids, which were significantly higher than monounsaturated fatty acids (19.54% ),of which linoleic acid content was the highest and was rich in oleic acid, eicosadienoic acid and eicosatrienoic acid.